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NSF antibody (AA 662-711)

This Rabbit Polyclonal antibody specifically detects NSF in WB. It exhibits reactivity toward Human, Rat, Mouse, Cow, Hamster, Pig, Rabbit, Horse and Zebrafish (Danio rerio).
Catalog No. ABIN6744481

Quick Overview for NSF antibody (AA 662-711) (ABIN6744481)

Target

See all NSF Antibodies
NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

Reactivity

  • 52
  • 32
  • 32
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Human, Rat, Mouse, Cow, Hamster, Pig, Rabbit, Horse, Zebrafish (Danio rerio)

Host

  • 63
  • 6
  • 1
Rabbit

Clonality

  • 66
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Polyclonal

Conjugate

  • 37
  • 5
  • 5
  • 4
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This NSF antibody is un-conjugated

Application

  • 58
  • 27
  • 13
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  • 12
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  • 11
  • 6
  • 5
  • 3
  • 1
  • 1
  • 1
Western Blotting (WB)
  • Binding Specificity

    • 15
    • 9
    • 6
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    • 3
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    • 1
    • 1
    • 1
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    AA 662-711

    Specificity

    Human NSF

    Predicted Reactivity

    Percent identity by BLAST analysis: Human, Mouse, Rat, Rabbit, Horse, Pig (100%) Dog (92%) Bovine, Chicken, Zebrafish (85%).

    Purification

    Immunoaffinity purified

    Immunogen

    Synthetic peptide located between aa662-711 of human NSF (P46459). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Rabbit, Horse, Pig (100%), Monkey, Dog, Opossum, Guinea pig (92%), Bovine, Turkey, Zebra finch, Chicken, Lizard, Zebrafish (85%).

    Type of Immunogen: Synthetic peptide
  • Application Notes

    Approved: WB (0.2 - 1 μg/mL)

    Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

    Comment

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Distilled water

    Concentration

    Lot specific

    Buffer

    Lyophilized from PBS with 2 % sucrose

    Handling Advice

    Avoid repeat freeze-thaw cycles.

    Storage

    4 °C,-20 °C

    Storage Comment

    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Target

    NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

    Alternative Name

    NSF

    Background

    Name/Gene ID: NSF

    Synonyms: NSF, NEM-sensitive fusion protein, SKD2, Vesicular-fusion protein NSF, Vesicle-fusing ATPase

    Gene ID

    4905

    UniProt

    P46459
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