ATF4 antibody (C-Term)

Catalog No. ABIN6744558

The Rabbit Polyclonal anti-ATF4 antibody (ABIN6744558) specifically detects ATF4 in WB. The antibody is reactive with Mouse, Rat, Cow, Guinea Pig, Sheep, Pig and Hamster samples.

Quick Overview for ATF4 antibody (C-Term) (ABIN6744558)

Target: ATF4 (Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4))

Reactivity: Mouse, Rat, Cow, Guinea Pig, Sheep, Pig, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATF4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ATF4 Antibody

Binding Specificity: C-Term

Specificity: Mouse ATF4

Predicted Reactivity: Percent identity by BLAST analysis: Mouse, Rat, Sheep, Bovine, Pig, Guinea pig (100%) Human, Dog (92%) Chicken (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of mouse Atf4 (Q5U4B2, NP_033846). Percent identity by BLAST analysis: Galago, Mouse, Rat, Sheep, Hamster, Bovine, Pig, Guinea pig (100%), Human, Gorilla, Gibbon, Monkey, Elephant, Panda, Dog (92%), Opossum, Turkey, Zebra finch, Chicken, Platypus (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ATF4

Target: ATF4 (Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4))

Alternative Name: ATF4

Background: Name/Gene ID: ATF4
Family: Transcription factor

Synonyms: ATF4, CREB-2, CREB2, TXREB, DNA-binding protein TAXREB67, TAXREB67

Gene ID: 468

NCBI Accession: NP_033846

UniProt: P18848

Pathways: Thyroid Hormone Synthesis, Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Unfolded Protein Response