MITF antibody (AA 121-170)

Catalog No. ABIN6744669

The Rabbit Polyclonal anti-MITF antibody has been validated for WB. It is suitable to detect MITF in samples from Mouse, Rat, Guinea Pig, Horse and Hamster.

Quick Overview for MITF antibody (AA 121-170) (ABIN6744669)

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Reactivity: Mouse, Rat, Guinea Pig, Horse, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MITF antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MITF Antibody

Binding Specificity: AA 121-170

Specificity: Mouse MITF

Predicted Reactivity: Percent identity by BLAST analysis: Mouse, Rat, Horse, Guinea pig (100%) Human, Dog, Bovine, Rabbit, Pig (92%) Chicken (91%) Xenopus (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa121-170 of mouse Mitf (Q32MU7, NP_032627). Percent identity by BLAST analysis: Mouse, Rat, Hamster, Horse, Guinea pig (100%), Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Elephant, Dog, Bovine, Bat, Rabbit, Pig, Lizard (92%), Turkey, Zebra finch, Chicken (91%), Opossum (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for MITF

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Alternative Name: MITF

Background: Name/Gene ID: MITF

Synonyms: MITF, BHLHe32, CMM8, MI, Waardenburg syndrome, type 2A, WS2, WS2A

Gene ID: 4286

NCBI Accession: NP_032627

UniProt: O75030

Pathways: Chromatin Binding