HFE antibody (AA 35-84)

Catalog No. ABIN6744769

This Rabbit Polyclonal antibody specifically detects HFE in WB. It exhibits reactivity toward Human, Mouse, Rabbit and Pig.

Quick Overview for HFE antibody (AA 35-84) (ABIN6744769)

Target: HFE (Hemochromatosis (HFE))

Reactivity: Human, Mouse, Rabbit, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HFE antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HFE Antibody

Binding Specificity: AA 35-84

Specificity: Human HFE

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Mouse, Pig (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa35-84 of human HFE (Q30201, NP_000401). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Marmoset (100%), Monkey (92%), Mouse, Elephant, Horse, Pig (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for HFE

Target: HFE (Hemochromatosis (HFE))

Alternative Name: HFE

Background: Name/Gene ID: HFE

Synonyms: HFE, HFE1, HH, MHC class I-like protein HFE, Hemochromatosis, High Fe, MVCD7, HLA-H, HLAH, TFQTL2

Gene ID: 3077

NCBI Accession: NP_000401

UniProt: Q30201

Pathways: Transition Metal Ion Homeostasis, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process