CHRM3 antibody (AA 481-589)

Catalog No. ABIN674484

Cited in 3+ publications. The Rabbit Polyclonal anti-CHRM3 antibody (ABIN674484) specifically detects CHRM3 in ELISA, WB, IHC (p), IF (cc), IF (p) and IHC (fro). The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for CHRM3 antibody (AA 481-589) (ABIN674484)

Target: CHRM3 (Cholinergic Receptor, Muscarinic 3 (CHRM3))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHRM3 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-CHRM3 Antibody

Binding Specificity: AA 481-589

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse Chrm3

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-CHRM3 antibody (ABIN674484)

Zhao, Yang, Chen, Wang, Zhang, Zhang: "Expression of M3 acetylcholine receptor in asthmatic mice and bronchial airway remodeling prediction." in: Genetics and molecular research : GMR, Vol. 15, Issue 3, (2016) (PubMed).

Ozgun-Acar, Celik-Turgut, Gazioglu, Kolak, Ozbal, Ergur, Arslan, Sen, Topcu: "Capparis ovata treatment suppresses inflammatory cytokine expression and ameliorates experimental allergic encephalomyelitis model of multiple sclerosis in C57BL/6 mice." in: Journal of neuroimmunology, Vol. 298, pp. 106-16, (2016) (PubMed).

Chen, Li, Zhang, Zhai, Gong, Qiang, Xue: "An ENU-induced mutation of Nrg1 causes dilated pupils and a reduction in muscarinic receptors in the sphincter pupillae." in: PLoS ONE, Vol. 6, Issue 9, pp. e25176, (2011) (PubMed).

Target Details for CHRM3

Target: CHRM3 (Cholinergic Receptor, Muscarinic 3 (CHRM3))

Alternative Name: Chrm3

Background:

Synonyms: M3, M3R, Chrm-3, Muscarinic acetylcholine receptor M3, Mm3 mAChR, Chrm3

Background: The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.

Gene ID: 12671

UniProt: Q9ERZ3

Pathways: Synaptic Membrane