EIF4A1 antibody (AA 215-264)

Catalog No. ABIN6744846

The Rabbit Polyclonal anti-EIF4A1 antibody has been validated for WB. It is suitable to detect EIF4A1 in samples from Human, Rat, Mouse, Cow, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Monkey, Pig and Xenopus laevis.

Quick Overview for EIF4A1 antibody (AA 215-264) (ABIN6744846)

Target: EIF4A1 (Eukaryotic Translation Initiation Factor 4A1 (EIF4A1))

Reactivity: Human, Rat, Mouse, Cow, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Monkey, Pig, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF4A1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EIF4A1 Antibody

Binding Specificity: AA 215-264

Specificity: Human EIF4A1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Rabbit, Horse, Pig, Guinea pig, Xenopus, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa215-264 of human EIF4A1 (P60842, NP_001407). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Platypus, Xenopus, Catfish, Stickleback, Zebrafish (100%), Hamster, Dog, Bat, Turkey, Zebra finch, Chicken, Nematode (92%), Slime mold (91%), Rice, Arabidopsis (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for EIF4A1

Target: EIF4A1 (Eukaryotic Translation Initiation Factor 4A1 (EIF4A1))

Alternative Name: EIF4A1 / EIF4A

Background: Name/Gene ID: EIF4A1

Synonyms: EIF4A1, EIF-4A-I, DDX2A, EIF4A, EIF4A-I, EIF-4A

Gene ID: 1973

NCBI Accession: NP_001407

UniProt: P60842