POLR1B antibody (AA 1007-1056)

Catalog No. ABIN6744853

The Rabbit Polyclonal anti-POLR1B antibody (ABIN6744853) specifically detects POLR1B in WB. The antibody is reactive with Human, Mouse, Pig, Rabbit, Zebrafish (Danio rerio), Bat and Monkey samples.

Quick Overview for POLR1B antibody (AA 1007-1056) (ABIN6744853)

Target: POLR1B (Polymerase (RNA) I Polypeptide B, 128kDa (POLR1B))

Reactivity: Human, Mouse, Pig, Rabbit, Zebrafish (Danio rerio), Bat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR1B antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-POLR1B Antibody

Binding Specificity: AA 1007-1056

Specificity: Human POLR1B

Predicted Reactivity: Percent identity by BLAST analysis: Human, Rabbit, Pig (100%) Rat, Dog, Bovine, Horse (92%) Mouse, Zebrafish (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa1007-1056 of human POLR1B (Q9H9Y6, NP_061887). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Bat, Rabbit, Pig (100%), Rat, Panda, Dog, Bovine, Horse, Opossum, Guinea pig (92%), Mouse, Elephant, Platypus, Xenopus, Stickleback, Zebrafish (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for POLR1B

Target: POLR1B (Polymerase (RNA) I Polypeptide B, 128kDa (POLR1B))

Alternative Name: POLR1B

Background: Name/Gene ID: POLR1B

Synonyms: POLR1B, RNA polymerase I subunit 2, RPA135, Rpo1-2

Gene ID: 84172

NCBI Accession: NP_061887

UniProt: Q9H9Y6