CYR61 antibody (AA 251-379)

Catalog No. ABIN674499

This anti-CYR61 antibody is a Rabbit Polyclonal antibody detecting CYR61 in WB and ELISA. Suitable for Human. Independently validated for use in Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections).

Quick Overview for CYR61 antibody (AA 251-379) (ABIN674499)

Target: CYR61 (Cysteine-Rich, Angiogenic Inducer, 61 (CYR61))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CYR61 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CYR61 Antibody

Binding Specificity: AA 251-379

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Dog,Cow,Sheep,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Cyr61

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000

Restrictions: For Research Use only

Independent Validation (IHC (fp))

Validation #100003 (Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections))

by: Memorial Sloan-Kettering Cancer Center, Parada lab

No.: #100003

Date: 10/15/2015

Antigen: CYR61

Method validated: Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections)

Positive Control: Tumor cell transplanted mouse brain was dissected

Notes: The staining pattern was as expected for Cyr61 as a secreted protein.

Validation Images

IF validation of CYR61 antibody ABIN674499 on mouse brain tumor sections. A. ABIN674499 diluted 1:100 (red), DAPI counterstaining (blue). B. Dilution factor 1:50. C. Dilution factor 1:100. D. Dilution factor 1:200.

Full Methods

Primary Antibody: ABIN674499

Secondary Antibody: Donkey-anti-Rabbit-Cy3

Full Protocol:

• Tumor cell transplanted mouse brain was dissected, fixed in 4% PFA for overnight, saturated in 30% sucrose, mounted in Cryo, and sectioned into 12µm sections.
• Immunostaining for Cryo Sections:
• Dry slides at 50-55°C for 15-30 min (or RT for 30min)
• Wash three times with PBS 5min (shake gently)
• Block with 10% Normal Donkey Serum (NDS) at RM for 1hour
• Incubate with primary antibody at 4°C overnight (Prepared in 2% NDS)
• Rabbit-anti-CYR61 antibody (ABIN674499) diluted 1:50-1:200
• Wash three times with PBS 5min (shake gently)
• Incubate with secondary antibody together with DAPI at RT for 1h (Prepared in 2% NDS), cover the chamber with foil
• Donkey-anti-Rabbit-Cy3
• DAPI 1:100
• Wash three times with PBS 5min (shake gently)
• Add three drops of water-based Immu-mount
• Cover with 24x60 cover glass
• Imaging
• Images were taken using a Zeiss Axio2-Imaging system, 20X-0.5NA-Dry Dye-1004-072 objective, DAPI and Cy3 channel.

Experimental Notes: ABIN674499 worked within the diution range 1:50 - 1:200

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for CYR61

Target: CYR61 (Cysteine-Rich, Angiogenic Inducer, 61 (CYR61))

Alternative Name: Cyr61

Background:

Synonyms: CCN1, GIG1, IGFBP10, Protein CYR61, CCN family member 1, Cysteine-rich angiogenic inducer 61, Insulin-like growth factor-binding protein 10, IBP-10, IGF-binding protein 10, IGFBP-10, Protein GIG1, CYR61

Background: Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3.

Gene ID: 3491

UniProt: O00622

Pathways: Positive Regulation of Endopeptidase Activity, Growth Factor Binding