ETF1 antibody (AA 35-84)

Catalog No. ABIN6745072

The Rabbit Polyclonal anti-ETF1 antibody has been validated for WB. It is suitable to detect ETF1 in samples from Human, Mouse, Rat, Cow, Dog, Zebrafish (Danio rerio), Guinea Pig, Horse, Rabbit, Xenopus laevis, Monkey, Pig, Hamster, Drosophila melanogaster, Chicken and Bat.

Quick Overview for ETF1 antibody (AA 35-84) (ABIN6745072)

Target: ETF1 (Eukaryotic Translation Termination Factor 1 (ETF1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Zebrafish (Danio rerio), Guinea Pig, Horse, Rabbit, Xenopus laevis, Monkey, Pig, Hamster, Drosophila melanogaster, Chicken, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ETF1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ETF1 Antibody

Binding Specificity: AA 35-84

Specificity: Human ETF1 / ERF1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Dog, Horse, Chicken, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa35-84 of human ETF1 (P62495, NP_004721). Percent identity by BLAST analysis: Human, Chimpanzee, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Chicken, Platypus, Lizard, Xenopus, Smelt, Stickleback, Zebrafish, Drosophila, Beetle, Grape, Tunicate (100%), Sea squirt, Rice, Arabidopsis (92%), Slime mold, Nematode (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ETF1

Target: ETF1 (Eukaryotic Translation Termination Factor 1 (ETF1))

Alternative Name: ETF1 / ERF1

Background: Name/Gene ID: ETF1

Synonyms: ETF1, D5S1995, ERF1, Eukaryotic release factor 1, Protein Cl1, RF1, SUP45L1, TB3-1

Gene ID: 2107

NCBI Accession: NP_004721

UniProt: P62495