CD3 epsilon antibody (AA 51-100)

Catalog No. ABIN6745374

The Rabbit Polyclonal anti-CD3 epsilon antibody has been validated for WB. It is suitable to detect CD3 epsilon in samples from Human.

Quick Overview for CD3 epsilon antibody (AA 51-100) (ABIN6745374)

Target: CD3 epsilon (CD3E)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD3 epsilon antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CD3 epsilon Antibody

Binding Specificity: AA 51-100

Specificity: Human CD3E

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa51-100 of human CD3E (P07766, NP_000724). Percent identity by BLAST analysis: Human, Gorilla (100%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.12 μg/mL)

Usage: Western Blot: Suggested dilution at 0.125 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: CD3E

Background: Name/Gene ID: CD3E

Synonyms: CD3E, CD3e antigen, CD3-epsilon, T-cell receptor epsilon chain, TCRE, T-cell receptors epsilon chain, T3E, CD3 Epsilon

Gene ID: 916

NCBI Accession: NP_000724

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway