Pyruvate Kinase, Muscle (PKM) (AA 391-440) antibody

Catalog No. ABIN6745581

This anti- antibody is a Rabbit Polyclonal antibody detecting in WB. Suitable for Human, Mouse, Rat, Xenopus laevis and Guinea Pig.

Quick Overview for Pyruvate Kinase, Muscle (PKM) (AA 391-440) antibody (ABIN6745581)

Target: Pyruvate Kinase, Muscle (PKM)

Reactivity: Human, Mouse, Rat, Xenopus laevis, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Product Details

Binding Specificity: AA 391-440

Specificity: Human PKM2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Rat (100%) Bovine, Horse, Pig, Chicken, Xenopus, Zebrafish (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa391-440 of human PKM (P11980, NP_002645). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Mouse, Rat, Guinea pig, Xenopus (100%), Marmoset, Elephant, Panda, Cat, Bovine, Rabbit, Horse, Pig, Turkey, Chicken, Salmon, Pufferfish, Zebrafish, Drosophila, Beetle (92%), Platypus (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details

Target: Pyruvate Kinase, Muscle (PKM)

Alternative Name: PKM / Pyruvate Kinase, Muscle

Background: Name/Gene ID: PKM

Synonyms: PKM, p58, PK3, PKM2, Pyruvate Kinase, Pyruvate kinase 2/3, Pyruvate kinase, m1, Pyruvate kinase, muscle, TCB, Tumor M2-PK, OIP-3, OIP3, Opa-interacting protein 3, CTHBP, PK, muscle type, PK2, Pyruvate kinase isozymes M1/M2, Pyruvate kinase muscle isozyme, THBP1

Gene ID: 5315

NCBI Accession: NP_002645

UniProt: P14618

Pathways: Warburg Effect