Nemo-Like Kinase antibody (AA 431-480)

Catalog No. ABIN6745669

The Rabbit Polyclonal anti-Nemo-Like Kinase antibody has been validated for WB. It is suitable to detect Nemo-Like Kinase in samples from Human, Mouse, Rat, Cow, Guinea Pig, Horse, Bat, Monkey and Pig.

Quick Overview for Nemo-Like Kinase antibody (AA 431-480) (ABIN6745669)

Target: Nemo-Like Kinase (NLK)

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Bat, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nemo-Like Kinase antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Nemo-Like Kinase Antibody

Binding Specificity: AA 431-480

Specificity: Human NLK

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Horse, Guinea pig (100%) Dog, Rabbit, Chicken, Xenopus, Zebrafish (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa431-480 of human NLK (Q9UBE8, NP_057315). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Bovine, Bat, Horse, Pig, Guinea pig (100%), Panda, Dog, Rabbit, Opossum, Turkey, Zebra finch, Chicken, Platypus, Xenopus, Pufferfish, Zebrafish (92%), Stickleback (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Nemo-Like Kinase

Target: Nemo-Like Kinase (NLK)

Alternative Name: NLK

Background: Name/Gene ID: NLK
Subfamily: MAPK
Family: Protein Kinase

Synonyms: NLK, LAK1, Nemo-like kinase, Nmo kinase, Nemo like kinase, Protein LAK1

Gene ID: 51701

NCBI Accession: NP_057315

UniProt: Q9UBE8

Pathways: Ubiquitin Proteasome Pathway