HSP90AA1 antibody (AA 108-157)

Catalog No. ABIN6745698

The Rabbit Polyclonal anti-HSP90AA1 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect HSP90AA1 in samples from Human, Mouse, Rat, Monkey, Rabbit, Guinea Pig, Pig and Hamster.

Quick Overview for HSP90AA1 antibody (AA 108-157) (ABIN6745698)

Target: HSP90AA1 (Heat Shock Protein 90kDa alpha (Cytosolic), Class A Member 1 (HSP90AA1))

Reactivity: Human, Mouse, Rat, Monkey, Rabbit, Guinea Pig, Pig, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSP90AA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HSP90AA1 Antibody

Binding Specificity: AA 108-157

Brand: IHC-plus™

Specificity: Human HSP90AA1 / Hsp90

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse (100%) Bovine (92%) Chicken (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa108-157 of human HSP90AA1 (P07900, NP_001017963). Percent identity by BLAST analysis: Human, Chimpanzee, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Rabbit, Pig, Guinea pig (100%), Gorilla, Sheep, Bovine, Bat, Horse (92%), Turkey, Zebra finch, Chicken (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Performed with MCF-7 cells, suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for HSP90AA1

Target: HSP90AA1 (Heat Shock Protein 90kDa alpha (Cytosolic), Class A Member 1 (HSP90AA1))

Alternative Name: HSP90AA1 / Hsp90 Alpha A1

Background: Name/Gene ID: HSP90AA1

Synonyms: HSP90AA1, EL52, HSP86, Hsp89, HSP89A, HSPC1, Heat shock 86 kDa, Hsp90, HSP90A, HSP90N, HSPCA, HSPCAL4, HSPN, LAP2, HSP 86, HSPCAL1

Gene ID: 3320

NCBI Accession: NP_001017963

UniProt: P07900

Pathways: M Phase, Regulation of Cell Size, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals