WT1 antibody (C-Term)

Catalog No. ABIN604665

This anti-WT1 antibody is a Rabbit Polyclonal antibody detecting WT1 in IHC, WB and IHC (p). Suitable for Human, Rat, Mouse, Rabbit, Dog, Monkey, Bat, Chicken and Xenopus laevis.

Quick Overview for WT1 antibody (C-Term) (ABIN604665)

Target: WT1 (Wilms Tumor 1 (WT1))

Reactivity: Human, Rat, Mouse, Rabbit, Dog, Monkey, Bat, Chicken, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WT1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-WT1 Antibody

Binding Specificity: C-Term

Specificity: Human WT1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Rabbit, Chicken, Xenopus (100%) Bovine, Horse, Pig, Zebrafish (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human WT1 (P19544, NP_077742). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bat, Rabbit, Opossum, Turkey, Zebra finch, Chicken, Platypus, Xenopus (100%), Bovine, Horse, Pig, Lizard, Salmon, Medaka, Zebrafish (92%), Pufferfish (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for WT1

Target: WT1 (Wilms Tumor 1 (WT1))

Alternative Name: WT1 / Wilms Tumor 1

Background: Name/Gene ID: WT1

Synonyms: WT1, GUD, EWS-WT1, Amino-terminal domain of EWS, WAGR, WIT-2, Wilms tumor protein, AWT1, Wilms tumor 1, WT33, NPHS4

Gene ID: 7490

NCBI Accession: NP_077742

UniProt: P19544

Pathways: Tube Formation