BACE1 antibody (AA 72-121)

Catalog No. ABIN6745737

The Rabbit Polyclonal anti-BACE1 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect BACE1 in samples from Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Monkey, Pig, Bat and Hamster.

Quick Overview for BACE1 antibody (AA 72-121) (ABIN6745737)

Target: BACE1 (Beta-secretase 1 (BACE1))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Monkey, Pig, Bat, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BACE1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-BACE1 Antibody

Binding Specificity: AA 72-121

Brand: IHC-plus™

Specificity: Human BACE1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Rabbit, Horse, Pig, Guinea pig (100%) Chicken (92%) Zebrafish (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa72-121 of human BACE1 (P56817, NP_036236). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (100%), Opossum, Turkey, Zebra finch, Chicken (92%), Zebrafish (90%), Platypus, Xenopus (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for BACE1

Target: BACE1 (Beta-secretase 1 (BACE1))

Alternative Name: BACE1 / BACE

Background: Name/Gene ID: BACE1
Subfamily: Aspartic A1
Family: Protease

Synonyms: BACE1, APP beta-secretase, Asp 2, ASP2, Aspartyl protease 2, Aspartylprotease 2, Beta-secretase, Beta-site APP-cleaving enzyme, BACE, HSPC104, KIAA1149, B-Secretase, Beta-secretase 1, Memapsin-2

Gene ID: 23621

NCBI Accession: NP_036236

UniProt: P56817