DBT antibody (AA 35-84)

Catalog No. ABIN6745750

This anti-DBT antibody is a Rabbit Polyclonal antibody detecting DBT in WB, IHC and IHC (p). Suitable for Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Monkey, Pig and Bat.

Quick Overview for DBT antibody (AA 35-84) (ABIN6745750)

Target: DBT (Dihydrolipoamide Branched Chain Transacylase E2 (DBT))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Monkey, Pig, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DBT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-DBT Antibody

Binding Specificity: AA 35-84

Brand: IHC-plus™

Specificity: Human DBT / E2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Rabbit, Horse, Pig, Guinea pig (100%) Chicken, Xenopus, Zebrafish (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa35-84 of human DBT (P11182, NP_001909). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (100%), Opossum, Turkey, Chicken, Platypus, Lizard, Xenopus, Zebrafish, Beetle (92%), Trout, Drosophila (91%), Stickleback, Sea squirt (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for DBT

Target: DBT (Dihydrolipoamide Branched Chain Transacylase E2 (DBT))

Alternative Name: DBT / E2

Background: Name/Gene ID: DBT

Synonyms: DBT, BCKAD E2 subunit, BCKAD-E2, BCKADE2, Dihydrolipoyl transacylase, E2, E2B, BCATE2

Gene ID: 1629

NCBI Accession: NP_001909

UniProt: P11182