ACAT1 antibody (AA 218-267)

Catalog No. ABIN6745752

The Rabbit Polyclonal anti-ACAT1 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect ACAT1 in samples from Human, Mouse, Rat, Dog, Guinea Pig, Horse, Rabbit, Monkey, Bat, Chicken, Hamster and Xenopus laevis.

Quick Overview for ACAT1 antibody (AA 218-267) (ABIN6745752)

Target: ACAT1 (Acetyl-CoA Acetyltransferase 1 (ACAT1))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Horse, Rabbit, Monkey, Bat, Chicken, Hamster, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACAT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ACAT1 Antibody

Binding Specificity: AA 218-267

Brand: IHC-plus™

Specificity: Human ACAT1

Predicted Reactivity: Percent identity by BLAST analysis: Mouse, Dog, Rabbit, Guinea pig, Chicken (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa218-267 of human ACAT1 (P35610, NP_000010). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bat, Rabbit, Horse, Opossum, Guinea pig, Turkey, Chicken, Xenopus (100%), Bovine (92%), Beetle (90%), Zebrafish (85%), Salmon, Smelt, Stickleback (84%), Drosophila (83%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ACAT1

Target: ACAT1 (Acetyl-CoA Acetyltransferase 1 (ACAT1))

Alternative Name: ACAT1

Background: Name/Gene ID: ACAT1

Synonyms: ACAT1, ACAT, Acetoacetyl-CoA thiolase, Acetyl-CoA acetyltransferase 1, MAT, T2, THIL

Gene ID: 38

NCBI Accession: NP_000010

UniProt: P24752