PAX7 antibody (AA 201-250)

Catalog No. ABIN6746020

This anti-PAX7 antibody is a Rabbit Polyclonal antibody detecting PAX7 in WB. Suitable for Human, Mouse, Rat, Zebrafish (Danio rerio), Chicken, Dog, Guinea Pig, Cow, Rabbit, Bat, Monkey and Xenopus laevis. This Primary Antibody has been cited in 3+ publications.

Quick Overview for PAX7 antibody (AA 201-250) (ABIN6746020)

Target: PAX7 (Paired Box 7 (PAX7))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Chicken, Dog, Guinea Pig, Cow, Rabbit, Bat, Monkey, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAX7 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PAX7 Antibody

Binding Specificity: AA 201-250

Specificity: Human PAX7

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Guinea pig, Chicken, Xenopus, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa201-250 of human Pax7 (NP_001178913). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Guinea pig, Turkey, Zebra finch, Chicken, Xenopus, Salmon, Zebrafish (100%), Goat, Horse, Platypus, Lizard, Stickleback (85%), Drosophila, Beetle (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Not recommended for: IF

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-PAX7 antibody (ABIN6746020)

Ku, Meier: "Phosphorylation of paxillin via the ERK mitogen-activated protein kinase cascade in EL4 thymoma cells." in: The Journal of biological chemistry, Vol. 275, Issue 15, pp. 11333-40, (2000) (PubMed).

Takayama, Tanaka, Nagai, Okada et al.: "Adenovirus-mediated overexpression of C-terminal Src kinase (Csk) in type I astrocytes interferes with cell spreading and attachment to fibronectin. Correlation with tyrosine phosphorylations of ..." in: The Journal of biological chemistry, Vol. 274, Issue 4, pp. 2291-7, (1999) (PubMed).

Salgia, Li, Lo, Brunkhorst, Kansas, Sobhany, Sun, Pisick, Hallek, Ernst: "Molecular cloning of human paxillin, a focal adhesion protein phosphorylated by P210BCR/ABL." in: The Journal of biological chemistry, Vol. 270, Issue 10, pp. 5039-47, (1995) (PubMed).

Target Details for PAX7

Target: PAX7 (Paired Box 7 (PAX7))

Alternative Name: PAX7

Background: Name/Gene ID: PAX7

Synonyms: PAX7, HUP1, Paired box 7, Paired box gene 7, Paired box homeotic gene 7, Paired box protein Pax-7, RMS2, Paired domain gene 7, PAX7 transcriptional factor, PAX7B

Gene ID: 5081

NCBI Accession: NP_001178913

UniProt: P23759