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CD28 antibody (AA 1-120)

Name: CD28 antibody (AA 1-120)
SKU: ABIN674604
Price: 384.62 USD
Availability: InStock

This anti-CD28 antibody is a Rabbit Polyclonal antibody detecting CD28 in FACS, WB, ELISA, IHC (fro), IF (p), IHC (p) and IF (cc). Suitable for Human, Mouse and Rat. This Primary Antibody has been cited in 1 publication.

Mouse splenocytes probed with Rabbit Anti-CD28 Polyclonal Antibody, Unconjugated . (CD28 antibody (AA 1-120))

FACS

Formalin-fixed and paraffin embedded rat colitis labeled with Anti-CD28 Polyclonal Antibody, Unconjugated (ABIN674604) at 1:200 followed by conjugation to the secondary antibody and DAB staining. (CD28 antibody (AA 1-120))

IHC

Formalin-fixed and paraffin embedded rat colitis labeled with Anti-CD28 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining. (CD28 antibody (AA 1-120))

IHC (p)

Paraformaldehyde-fixed, paraffin embedded Human rectal cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6. (CD28 antibody (AA 1-120))

IHC (p)

Formalin-fixed and paraffin embedded mouse lung labeled with Anti-CD28 Polyclonal Antibody, Unconjugated (ABIN674604) at 1:200 followed by conjugation to the secondary antibody and DAB staining (CD28 antibody (AA 1-120))

IHC

Formalin-fixed and paraffin-embedded rat colitis labeled with Anti-CD28 Polyclonal Antibody, Unconjugated (ABIN674604) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C. (CD28 antibody (AA 1-120))

Paraformaldehyde-fixed, paraffin embedded Human stomach, Antigen retrieval by boiling in sodium citrate buffer (pH6. (CD28 antibody (AA 1-120))

IHC (p)

7 images

(1 reference)

Catalog No. ABIN674604

Quick Overview for CD28 antibody (AA 1-120) (ABIN674604)

Target

See all CD28 Antibodies

CD28

Reactivity

Human, Mouse, Rat

Host

Rabbit

Clonality

Polyclonal

Conjugate

This CD28 antibody is un-conjugated

Application

Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Binding Specificity
15

15

8

5

5

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1
AA 1-120

Cross-Reactivity

Human, Mouse, Rat

Predicted Reactivity

Dog,Cow,Pig,Horse,Chicken

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from mouse CD28

Isotype

IgG
anti-CD28 (CD28) antibody
CD28 Reactivity: Human, Non-Human Primate FACS, WB, IP, IHC (fro), ICC Host: Mouse Monoclonal CD28-2 unconjugated

anti-CD28 (CD28) antibody (FITC)
CD28 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal CD28-2 FITC

anti-CD28 (CD28) antibody
CD28 Reactivity: Human, Non-Human Primate FACS, WB, IP, Func, IHC (fro), ICC Host: Mouse Monoclonal CD28-2 unconjugated

anti-CD28 (CD28) (AA 19-152) antibody
CD28 Reactivity: Human FACS, ELISA Host: Rabbit Monoclonal DM61 unconjugated Recombinant Antibody

anti-CD28 (CD28) antibody
CD28 Reactivity: Rat FACS, IP Host: Mouse Monoclonal JJ319 unconjugated

anti-CD28 (CD28) antibody (PE)
CD28 Reactivity: Rat FACS Host: Mouse Monoclonal JJ319 PE

anti-CD28 (CD28) (C-Term) antibody
CD28 Reactivity: Human, Mouse, Rat WB, IHC Host: Rabbit Polyclonal unconjugated

anti-CD28 (CD28) antibody (PE)
CD28 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal CD28-2 PE

anti-CD28 (CD28) antibody (APC)
CD28 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal CD28-2 APC

anti-CD28 (CD28) antibody (PerCP)
CD28 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal CD28-2 PerCP

Application Notes

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Liu, He, Chen, Ding, Geng, Wu, Liu, Wang: "Costimulatory molecule CD28 participates in the process of embryo implantation in mice." in: Reproductive sciences (Thousand Oaks, Calif.), Vol. 21, Issue 6, pp. 686-95, (2014) (PubMed).
Target

CD28

Alternative Name

CD28

Background

Synonyms: T-cell-specific surface glycoprotein CD28, CD28
Background: Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.

Gene ID

12487

UniProt

P31041

Pathways

TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response