T Brachyury Protein (N-Term) antibody

Catalog No. ABIN6746059

This anti- antibody is a Rabbit Polyclonal antibody detecting in WB. Suitable for Human, Mouse, Cow, Dog, Rat, Guinea Pig, Horse, Zebrafish (Danio rerio), Monkey, Pig, Chicken and Xenopus laevis. This Primary Antibody has been cited in 2+ publications.

Quick Overview for T Brachyury Protein (N-Term) antibody (ABIN6746059)

Target: T Brachyury Protein

Reactivity: Human, Mouse, Cow, Dog, Rat, Guinea Pig, Horse, Zebrafish (Danio rerio), Monkey, Pig, Chicken, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Product Details

Binding Specificity: N-Term

Specificity: Human T / Brachyury

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Pig, Guinea pig, Chicken, Xenopus (100%) Dog, Rabbit (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human T (O15178, NP_003172). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Tamarin, Mouse, Rat, Elephant, Panda, Dog, Bovine, Horse, Pig, Opossum, Guinea pig, Zebra finch, Sparrow, Chicken, Platypus, Xenopus, Newt, Stickleback, Zebrafish (100%), Bat, Rabbit (92%), Lizard (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References

Suhr, Senut, Whitelegge, Faull, Cuizon, Gage: "Identities of sequestered proteins in aggregates from cells with induced polyglutamine expression." in: The Journal of cell biology, Vol. 153, Issue 2, pp. 283-94, (2001) (PubMed).

Hunt, Morimoto: "Conserved features of eukaryotic hsp70 genes revealed by comparison with the nucleotide sequence of human hsp70." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 82, Issue 19, pp. 6455-9, (1985) (PubMed).

Target Details

Target: T Brachyury Protein

Alternative Name: T / Brachyury

Background: Name/Gene ID: T / Brachyury

Synonyms: T / Brachyury, Brachyury, Brachyury protein, Protein T, TFT, T, T brachyury (mouse) homolog, T brachyury homolog, T, brachyury homolog (mouse)

Gene ID: 6862

NCBI Accession: NP_003172

UniProt: O15178

Pathways: Tube Formation