T Brachyury Protein (N-Term) antibody

Catalog No. ABIN6746059

Cited in 2+ publications. The Rabbit Polyclonal anti-T Brachyury Protein antibody (ABIN6746059) specifically detects T Brachyury Protein in WB. The antibody is reactive with Human, Mouse, Cow, Dog, Rat, Guinea Pig, Horse, Zebrafish (Danio rerio), Monkey, Pig, Chicken and Xenopus laevis samples.

Quick Overview for T Brachyury Protein (N-Term) antibody (ABIN6746059)

Target: T Brachyury Protein

Reactivity: Human, Mouse, Cow, Dog, Rat, Guinea Pig, Horse, Zebrafish (Danio rerio), Monkey, Pig, Chicken, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Product Details

Binding Specificity: N-Term

Specificity: Human T / Brachyury

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Pig, Guinea pig, Chicken, Xenopus (100%) Dog, Rabbit (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human T (O15178, NP_003172). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Tamarin, Mouse, Rat, Elephant, Panda, Dog, Bovine, Horse, Pig, Opossum, Guinea pig, Zebra finch, Sparrow, Chicken, Platypus, Xenopus, Newt, Stickleback, Zebrafish (100%), Bat, Rabbit (92%), Lizard (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References

Suhr, Senut, Whitelegge, Faull, Cuizon, Gage: "Identities of sequestered proteins in aggregates from cells with induced polyglutamine expression." in: The Journal of cell biology, Vol. 153, Issue 2, pp. 283-94, (2001) (PubMed).

Hunt, Morimoto: "Conserved features of eukaryotic hsp70 genes revealed by comparison with the nucleotide sequence of human hsp70." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 82, Issue 19, pp. 6455-9, (1985) (PubMed).

Target Details

Target: T Brachyury Protein

Alternative Name: T / Brachyury

Background: Name/Gene ID: T / Brachyury

Synonyms: T / Brachyury, Brachyury, Brachyury protein, Protein T, TFT, T, T brachyury (mouse) homolog, T brachyury homolog, T, brachyury homolog (mouse)

Gene ID: 6862

NCBI Accession: NP_003172

UniProt: O15178

Pathways: Tube Formation