Midline 2 antibody (AA 210-259)

Catalog No. ABIN6746086

This Rabbit Polyclonal antibody specifically detects Midline 2 in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Rabbit, Guinea Pig, Horse, Pig, Monkey, Bat and Hamster.

Quick Overview for Midline 2 antibody (AA 210-259) (ABIN6746086)

Target: Midline 2 (MID2)

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Guinea Pig, Horse, Pig, Monkey, Bat, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Midline 2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Midline 2 Antibody

Binding Specificity: AA 210-259

Specificity: Mouse MID2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Rabbit, Horse, Pig, Guinea pig (100%) Chicken (90%) Xenopus (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa210-259 of mouse Mid2 (B1AVF4, NP_035975). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bat, Rabbit, Horse, Pig, Guinea pig (100%), Opossum, Turkey, Zebra finch (92%), Chicken (90%), Xenopus (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Midline 2

Target: Midline 2 (MID2)

Alternative Name: TRIM1 / MID2

Background: Name/Gene ID: MID2

Synonyms: MID2, FXY2, Midin-2, Midline-2, Midin 2, Midline 2, Midline defect 2, RNF60, RING finger protein 60, TRIM1, Tripartite motif protein 1

Gene ID: 11043

NCBI Accession: NP_035975

UniProt: Q9UJV3