Amylin/DAP antibody (N-Term)

Catalog No. ABIN6746093

The Rabbit Polyclonal anti-Amylin/DAP antibody has been validated for WB. It is suitable to detect Amylin/DAP in samples from Human, Rabbit, Dog and Monkey. There are 2+ publications available.

Quick Overview for Amylin/DAP antibody (N-Term) (ABIN6746093)

Target: Amylin/DAP (IAPP) (Islet Amyloid Polypeptide (IAPP))

Reactivity: Human, Rabbit, Dog, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Amylin/DAP antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Amylin/DAP Antibody

Binding Specificity: N-Term

Specificity: Human IAPP

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Dog (85%) Rabbit (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human IAPP (P10997, NP_000406). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Baboon, Cat, Horse (92%), Panda, Dog (85%), Rabbit (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 50 μL sterile ddH2O.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-Amylin/DAP antibody (ABIN6746093)

Wang, Reddy, Shen: "Higher order arrangement of the eukaryotic nuclear bodies." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 21, pp. 13583-8, (2002) (PubMed).

Cifuentes-Diaz, Frugier, Tiziano, Lacène, Roblot, Joshi, Moreau, Melki: "Deletion of murine SMN exon 7 directed to skeletal muscle leads to severe muscular dystrophy." in: The Journal of cell biology, Vol. 152, Issue 5, pp. 1107-14, (2001) (PubMed).

Target Details for Amylin/DAP

Target: Amylin/DAP (IAPP) (Islet Amyloid Polypeptide (IAPP))

Alternative Name: IAPP / Amylin

Background: Name/Gene ID: IAPP

Synonyms: IAPP, Diabetes-associated peptide, Islet amyloid polypeptide, AMYLIN, IAP, Insulinoma amyloid peptide

Gene ID: 3375

NCBI Accession: NP_000406

UniProt: P10997

Pathways: Hormone Activity, Feeding Behaviour