STEAP1 antibody (AA 106-155)

Catalog No. ABIN6746136

Cited in 2+ publications. The Rabbit Polyclonal anti-STEAP1 antibody (ABIN6746136) specifically detects STEAP1 in WB. The antibody is reactive with Human, Rat, Mouse, Monkey and Pig samples.

Quick Overview for STEAP1 antibody (AA 106-155) (ABIN6746136)

Target: STEAP1 (Six Transmembrane Epithelial Antigen of The Prostate 1 (STEAP1))

Reactivity: Human, Rat, Mouse, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STEAP1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-STEAP1 Antibody

Binding Specificity: AA 106-155

Specificity: Human STEAP1

Predicted Reactivity: Percent identity by BLAST analysis: Pig (100%) Human, Bovine, Rabbit (92%) Mouse, Rat (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa106-155 of human STEAP1 (Q9UHE8, NP_036581). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Baboon, Monkey, Marmoset, Pig (100%), Bovine, Rabbit, Horse, Guinea pig (92%), Galago, Mouse, Rat, Hamster, Bat, Armadillo (85%), Elephant (84%), Opossum (81%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-STEAP1 antibody (ABIN6746136)

Howe, Hogan, Juliano: "Regulation of vasodilator-stimulated phosphoprotein phosphorylation and interaction with Abl by protein kinase A and cell adhesion." in: The Journal of biological chemistry, Vol. 277, Issue 41, pp. 38121-6, (2002) (PubMed).

Drees, Friederich, Fradelizi, Louvard, Beckerle, Golsteyn: "Characterization of the interaction between zyxin and members of the Ena/vasodilator-stimulated phosphoprotein family of proteins." in: The Journal of biological chemistry, Vol. 275, Issue 29, pp. 22503-11, (2000) (PubMed).

Target Details for STEAP1

Target: STEAP1 (Six Transmembrane Epithelial Antigen of The Prostate 1 (STEAP1))

Alternative Name: STEAP1 / STEAP

Background: Name/Gene ID: STEAP1

Synonyms: STEAP1, Metalloreductase STEAP1, PRSS24, STEAP

Gene ID: 26872

NCBI Accession: NP_036581

UniProt: Q9UHE8

Pathways: Transition Metal Ion Homeostasis