NFIA antibody (C-Term)

Catalog No. ABIN6746156

The Rabbit Polyclonal anti-NFIA antibody has been validated for WB. It is suitable to detect NFIA in samples from Human, Mouse, Rat, Cow, Horse, Rabbit, Monkey and Xenopus laevis. There are 3+ publications available.

Quick Overview for NFIA antibody (C-Term) (ABIN6746156)

Target: NFIA (Nuclear Factor I/A (NFIA))

Reactivity: Human, Mouse, Rat, Cow, Horse, Rabbit, Monkey, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFIA antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NFIA Antibody

Binding Specificity: C-Term

Specificity: Mouse NFIA

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Rabbit, Horse (100%) Guinea pig, Chicken (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of mouse Nfia (NP_035035). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Rabbit, Horse, Xenopus (100%), Galago, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-NFIA antibody (ABIN6746156)

Suzuki, Ishizaka, Tsukamoto, Minami, Taguchi, Nagai, Ohno: "Pressurization facilitates adenovirus-mediated gene transfer into vein graft." in: FEBS letters, Vol. 470, Issue 3, pp. 370-4, (2000) (PubMed).

Foresti, Clark, Green, Motterlini: "Thiol compounds interact with nitric oxide in regulating heme oxygenase-1 induction in endothelial cells. Involvement of superoxide and peroxynitrite anions." in: The Journal of biological chemistry, Vol. 272, Issue 29, pp. 18411-7, (1997) (PubMed).

Ishikawa, Navab, Leitinger, Fogelman, Lusis: "Induction of heme oxygenase-1 inhibits the monocyte transmigration induced by mildly oxidized LDL." in: The Journal of clinical investigation, Vol. 100, Issue 5, pp. 1209-16, (1997) (PubMed).

Target Details for NFIA

Target: NFIA (Nuclear Factor I/A (NFIA))

Alternative Name: NFIA / Nuclear Factor 1

Background: Name/Gene ID: NFIA

Synonyms: NFIA, CTF, NF1-A, NF-I/A, NFI-L, Nuclear Factor 1, TGGCA-binding protein, NFI-A, Nuclear factor 1 A-type, KIAA1439, Nuclear factor 1/A, Nuclear factor I/A

Gene ID: 4774

NCBI Accession: NP_035035

UniProt: Q12857