TCEA1 antibody (C-Term)

Catalog No. ABIN6746165

The Rabbit Polyclonal anti-TCEA1 antibody has been validated for WB. It is suitable to detect TCEA1 in samples from Human, Mouse, Rat, Dog, Guinea Pig, Horse, Rabbit, Cow, Zebrafish (Danio rerio), Monkey, Pig, Bat, Chicken, Xenopus laevis, Drosophila melanogaster and Hamster. There are 4+ publications available.

Quick Overview for TCEA1 antibody (C-Term) (ABIN6746165)

Target: TCEA1 (Transcription Elongation Factor A Protein 1 (TCEA1))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Horse, Rabbit, Cow, Zebrafish (Danio rerio), Monkey, Pig, Bat, Chicken, Xenopus laevis, Drosophila melanogaster, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TCEA1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TCEA1 Antibody

Binding Specificity: C-Term

Specificity: Rat TCEA1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Guinea pig, Chicken, Xenopus, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of rat Tcea1 (Q4KLL0, NP_001020906). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Baboon, Monkey, Marmoset, Mouse, Rat, Shrew, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Armadillo, Platypus, Xenopus, Frog, Trout, Catfish, Salmon, Sablefish, Stickleback, Pike, Zebrafish, Drosophila, Rice, Grape (100%), Goat, Sea squirt, Beetle (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-TCEA1 antibody (ABIN6746165)

Stenmark, Vitale, Ullrich, Zerial: "Rabaptin-5 is a direct effector of the small GTPase Rab5 in endocytic membrane fusion." in: Cell, Vol. 83, Issue 3, pp. 423-32, (1996) (PubMed).

Ullrich, Horiuchi, Bucci, Zerial: "Membrane association of Rab5 mediated by GDP-dissociation inhibitor and accompanied by GDP/GTP exchange." in: Nature, Vol. 368, Issue 6467, pp. 157-60, (1994) (PubMed).

Li, Stahl: "Structure-function relationship of the small GTPase rab5." in: The Journal of biological chemistry, Vol. 268, Issue 32, pp. 24475-80, (1993) (PubMed).

Sanford, Pan, Wessling-Resnick: "Prenylation of Rab5 is dependent on guanine nucleotide binding." in: The Journal of biological chemistry, Vol. 268, Issue 32, pp. 23773-6, (1993) (PubMed).

Target Details for TCEA1

Target: TCEA1 (Transcription Elongation Factor A Protein 1 (TCEA1))

Alternative Name: TCEA1 / TFIIS

Background: Name/Gene ID: TCEA1

Synonyms: TCEA1, GTF2S, SII, TCEA, TFIIS

Gene ID: 6917

NCBI Accession: NP_001020906

UniProt: P23193