RNF183 antibody (AA 44-93)

Catalog No. ABIN6746213

This Rabbit Polyclonal antibody specifically detects RNF183 in WB. It exhibits reactivity toward Human, Mouse, Cow, Guinea Pig, Rabbit, Rat, Dog and Monkey and has been mentioned in 2+ publications.

Quick Overview for RNF183 antibody (AA 44-93) (ABIN6746213)

Target: RNF183 (Ring Finger Protein 183 (RNF183))

Reactivity: Human, Mouse, Cow, Guinea Pig, Rabbit, Rat, Dog, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF183 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RNF183 Antibody

Binding Specificity: AA 44-93

Specificity: Mouse RNF183

Predicted Reactivity: Percent identity by BLAST analysis: Rat, Dog, Guinea pig (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa44-93 of mouse Rnf183 (Q8QZS5, NP_705724). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Rabbit, Guinea pig (100%), Horse (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-RNF183 antibody (ABIN6746213)

Araki, Zimonjic, Popescu, Milbrandt: "Mechanism of homophilic binding mediated by ninjurin, a novel widely expressed adhesion molecule." in: The Journal of biological chemistry, Vol. 272, Issue 34, pp. 21373-80, (1997) (PubMed).

Araki, Milbrandt: "Ninjurin, a novel adhesion molecule, is induced by nerve injury and promotes axonal growth." in: Neuron, Vol. 17, Issue 2, pp. 353-61, (1996) (PubMed).

Target Details for RNF183

Target: RNF183 (Ring Finger Protein 183 (RNF183))

Alternative Name: RNF183

Background: Name/Gene ID: RNF183

Synonyms: RNF183, Ring finger protein 183

Gene ID: 138065

NCBI Accession: NP_705724

UniProt: Q96D59