SDC3 antibody (AA 27-76)

Catalog No. ABIN6746281

This Rabbit Polyclonal antibody specifically detects SDC3 in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Guinea Pig, Horse, Rabbit, Bat, Monkey, Pig and Chicken and has been mentioned in 2+ publications.

Quick Overview for SDC3 antibody (AA 27-76) (ABIN6746281)

Target: SDC3 (Syndecan 3 (SDC3))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Horse, Rabbit, Bat, Monkey, Pig, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SDC3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SDC3 Antibody

Binding Specificity: AA 27-76

Specificity: Mouse SDC3 / Syndecan 3

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Rabbit, Pig, Guinea pig, Chicken (100%) Xenopus (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa27-76 of mouse Sdc3 (Q64519, NP_035650). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Zebra finch, Chicken (100%), Xenopus (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-SDC3 antibody (ABIN6746281)

Hagglund, Van Sant, Lopez, Roizman: "Herpes simplex virus 1-infected cell protein 0 contains two E3 ubiquitin ligase sites specific for different E2 ubiquitin-conjugating enzymes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 2, pp. 631-6, (2002) (PubMed).

Shimura, Schlossmacher, Hattori, Frosch, Trockenbacher, Schneider, Mizuno, Kosik, Selkoe: "Ubiquitination of a new form of alpha-synuclein by parkin from human brain: implications for Parkinson's disease." in: Science (New York, N.Y.), Vol. 293, Issue 5528, pp. 263-9, (2001) (PubMed).

Target Details for SDC3

Target: SDC3 (Syndecan 3 (SDC3))

Alternative Name: SDC3 / Syndecan 3

Background: Name/Gene ID: SDC3

Synonyms: SDC3, KIAA0468, N-syndecan, SDCN, SYND3, Syndecan 3, Syndecan-3, Syndecan 3 (N-syndecan), Syndecan neural type, Syndecan proteoglycan 3

Gene ID: 9672

NCBI Accession: NP_035650

UniProt: O75056

Pathways: Glycosaminoglycan Metabolic Process