ATP5F1 antibody (AA 45-94)

Catalog No. ABIN6746288

The Rabbit Polyclonal anti-ATP5F1 antibody has been validated for WB. It is suitable to detect ATP5F1 in samples from Human, Mouse, Rat, Cow, Dog, Horse, Pig and Monkey.

Quick Overview for ATP5F1 antibody (AA 45-94) (ABIN6746288)

Target: ATP5F1 (ATP Synthase, H+ Transporting, Mitochondrial Fo Complex, Subunit B1 (ATP5F1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Pig, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP5F1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ATP5F1 Antibody

Binding Specificity: AA 45-94

Specificity: Mouse ATP5F1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Dog, Pig (100%) Chicken, Xenopus, Zebrafish (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa45-94 of mouse Atp5f1 (Q9CQQ7, NP_033855). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Horse, Pig, Opossum, Platypus, Catfish, Salmon (100%), Rabbit, Guinea pig, Turkey, Zebra finch, Chicken, Xenopus, Sablefish, Stickleback, Pike, Zebrafish (92%), Lizard (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ATP5F1

Target: ATP5F1 (ATP Synthase, H+ Transporting, Mitochondrial Fo Complex, Subunit B1 (ATP5F1))

Alternative Name: ATP5F1

Background: Name/Gene ID: ATP5F1
Subfamily: ATPase - F type
Family: Transporter

Synonyms: ATP5F1, ATPase subunit b, H+-ATP synthase subunit b, PIG47

Gene ID: 515

NCBI Accession: NP_033855

UniProt: P24539

Pathways: Proton Transport, Ribonucleoside Biosynthetic Process