CORO2B antibody (AA 275-324)

Catalog No. ABIN6746343

This Rabbit Polyclonal antibody specifically detects CORO2B in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Pig, Bat and Monkey and has been mentioned in 3+ publications.

Quick Overview for CORO2B antibody (AA 275-324) (ABIN6746343)

Target: CORO2B (Coronin, Actin Binding Protein, 2B (CORO2B))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Pig, Bat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CORO2B antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CORO2B Antibody

Binding Specificity: AA 275-324

Specificity: Mouse CORO2B

Predicted Reactivity: Percent identity by BLAST analysis: Mouse, Rat, Bovine, Rabbit, Horse, Pig, Guinea pig (100%) Dog (92%) Chicken (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa275-324 of mouse Coro2b (NP_780693). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (100%), Panda, Dog, Turkey, Chicken (92%), Opossum, Zebra finch, Platypus (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-CORO2B antibody (ABIN6746343)

Saitoh, Pizzi, Wang: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, (2002) (PubMed).

Christmann, Kaina: "Nuclear translocation of mismatch repair proteins MSH2 and MSH6 as a response of cells to alkylating agents." in: The Journal of biological chemistry, Vol. 275, Issue 46, pp. 36256-62, (2000) (PubMed).

Palombo, Gallinari, Iaccarino, Lettieri, Hughes, DArrigo, Truong, Hsuan, Jiricny: "GTBP, a 160-kilodalton protein essential for mismatch-binding activity in human cells." in: Science (New York, N.Y.), Vol. 268, Issue 5219, pp. 1912-4, (1995) (PubMed).

Target Details for CORO2B

Target: CORO2B (Coronin, Actin Binding Protein, 2B (CORO2B))

Alternative Name: CORO2B

Background: Name/Gene ID: CORO2B

Synonyms: CORO2B, Coronin-like protein C, Clipin-C, Coronin-2B, Clipin C, CLIPINC, KIAA0925, Protein FC96

Gene ID: 10391

NCBI Accession: NP_780693

UniProt: Q9UQ03