ARFIP1 antibody (C-Term)

Catalog No. ABIN6746382

This Rabbit Polyclonal antibody specifically detects ARFIP1 in WB. It exhibits reactivity toward Human, Rat, Cow, Dog, Horse, Rabbit, Guinea Pig, Bat, Monkey and Pig and has been mentioned in 3+ publications.

Quick Overview for ARFIP1 antibody (C-Term) (ABIN6746382)

Target: ARFIP1 (ADP-Ribosylation Factor Interacting Protein 1 (ARFIP1))

Reactivity: Human, Rat, Cow, Dog, Horse, Rabbit, Guinea Pig, Bat, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARFIP1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ARFIP1 Antibody

Binding Specificity: C-Term

Specificity: Human ARFIP1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Rat, Dog, Bovine, Rabbit, Horse (100%) Mouse, Chicken, Xenopus, Zebrafish (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human ARFIP1 (P53367, NP_001020766). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Galago, Marmoset, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Platypus, Lizard (100%), Gorilla, Mouse, Sheep, Chicken, Xenopus, Zebrafish (92%), Stickleback (85%), Salmon (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-ARFIP1 antibody (ABIN6746382)

Chirala, Huang, Jayakumar, Sakai, Wakil: "Animal fatty acid synthase: functional mapping and cloning and expression of the domain I constituent activities." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 11, pp. 5588-93, (1997) (PubMed).

Jayakumar, Tai, Huang, al-Feel, Hsu, Abu-Elheiga, Chirala, Wakil: "Human fatty acid synthase: properties and molecular cloning." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 19, pp. 8695-9, (1995) (PubMed).

Smith: "The animal fatty acid synthase: one gene, one polypeptide, seven enzymes." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 8, Issue 15, pp. 1248-59, (1995) (PubMed).

Target Details for ARFIP1

Target: ARFIP1 (ADP-Ribosylation Factor Interacting Protein 1 (ARFIP1))

Alternative Name: ARFIP1

Background: Name/Gene ID: ARFIP1

Synonyms: ARFIP1, Arfaptin-1, Arfaptin 1, HSU52521

Gene ID: 27236

NCBI Accession: NP_001020766

UniProt: P53367