MED19 antibody (AA 68-117)

Catalog No. ABIN6746410

This Rabbit Polyclonal antibody specifically detects MED19 in WB. It exhibits reactivity toward Human, Rat, Mouse, Cow, Dog, Horse, Rabbit, Pig, Bat, Chicken, Hamster and Monkey and has been mentioned in 2+ publications.

Quick Overview for MED19 antibody (AA 68-117) (ABIN6746410)

Target: MED19 (Mediator Complex Subunit 19 (MED19))

Reactivity: Human, Rat, Mouse, Cow, Dog, Horse, Rabbit, Pig, Bat, Chicken, Hamster, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED19 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MED19 Antibody

Binding Specificity: AA 68-117

Specificity: Rat MED19

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Chicken (100%) Guinea pig (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa68-117 of rat Med19 (D3ZAP1, NP_001101211). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Galago, Marmoset, Mouse, Rat, Hamster, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Chicken (100%), Elephant, Guinea pig, Lizard (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-MED19 antibody (ABIN6746410)

Stengelin, Hoffman: "Na,K-ATPase subunit isoforms in human reticulocytes: evidence from reverse transcription-PCR for the presence of alpha1, alpha3, beta2, beta3, and gamma." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 11, pp. 5943-8, (1997) (PubMed).

Hernando, Martin-Vasallo, Ghosh, Ghosh, Swaroop, Coca-Prados: "Nucleotide sequence of a cDNA for the beta 2 subunit isoform of Na+,K(+)-ATPase from human retina." in: Biochimica et biophysica acta, Vol. 1189, Issue 1, pp. 109-11, (1994) (PubMed).

Target Details for MED19

Target: MED19 (Mediator Complex Subunit 19 (MED19))

Alternative Name: MED19

Background: Name/Gene ID: MED19

Synonyms: MED19, DT2P1G7, LCMR1, Mediator complex subunit 19

Gene ID: 219541

NCBI Accession: NP_001101211

Pathways: Regulation of Lipid Metabolism by PPARalpha