PPP2R5E antibody (AA 35-84)

Catalog No. ABIN6746458

The Rabbit Polyclonal anti-PPP2R5E antibody has been validated for WB. It is suitable to detect PPP2R5E in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Bat, Chicken, Hamster, Monkey, Xenopus laevis and Pig.

Quick Overview for PPP2R5E antibody (AA 35-84) (ABIN6746458)

Target: PPP2R5E (Protein Phosphatase 2, Regulatory Subunit B', epsilon Isoform (PPP2R5E))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Bat, Chicken, Hamster, Monkey, Xenopus laevis, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPP2R5E antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PPP2R5E Antibody

Binding Specificity: AA 35-84

Specificity: Human PPP2R5E

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Horse, Pig, Guinea pig, Chicken, Xenopus, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa35-84 of human PPP2R5E (Q16537, NP_006237). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus, Salmon, Zebrafish (100%), Stickleback (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PPP2R5E

Target: PPP2R5E (Protein Phosphatase 2, Regulatory Subunit B', epsilon Isoform (PPP2R5E))

Alternative Name: PPP2R5E

Background: Name/Gene ID: PPP2R5E

Synonyms: PPP2R5E, PP2A, B subunit, R5 epsilon, PP2A, B subunit, B epsilon, PP2A, B subunit, B56 epsilon, PP2A, B subunit, PR61 epsilon

Gene ID: 5529

NCBI Accession: NP_006237

UniProt: Q16537

Pathways: PI3K-Akt Signaling