RAD21 antibody (AA 373-422)

Catalog No. ABIN6746464

The Rabbit Polyclonal anti-RAD21 antibody has been validated for WB. It is suitable to detect RAD21 in samples from Human, Mouse, Rat, Cow, Rabbit, Dog, Horse, Monkey, Guinea Pig, Chicken, Bat, Pig and Xenopus laevis. There are 2+ publications available.

Quick Overview for RAD21 antibody (AA 373-422) (ABIN6746464)

Target: RAD21 (RAD21 Homolog (RAD21))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Dog, Horse, Monkey, Guinea Pig, Chicken, Bat, Pig, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD21 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RAD21 Antibody

Binding Specificity: AA 373-422

Specificity: Rat RAD21

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Guinea pig, Chicken, Xenopus (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa373-422 of rat Rad21 (Q4KLH7, NP_001020872). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus (100%), Salmon, Medaka, Zebrafish (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-RAD21 antibody (ABIN6746464)

Jia, Patwari, Srinivasula, Newland, Fernandes-Alnemri, Alnemri, Kelsey: "Bax translocation is crucial for the sensitivity of leukaemic cells to etoposide-induced apoptosis." in: Oncogene, Vol. 20, Issue 35, pp. 4817-26, (2001) (PubMed).

Hatai, Matsuzawa, Inoshita, Mochida, Kuroda, Sakamaki, Kuida, Yonehara, Ichijo, Takeda: "Execution of apoptosis signal-regulating kinase 1 (ASK1)-induced apoptosis by the mitochondria-dependent caspase activation." in: The Journal of biological chemistry, Vol. 275, Issue 34, pp. 26576-81, (2000) (PubMed).

Target Details for RAD21

Target: RAD21 (RAD21 Homolog (RAD21))

Alternative Name: RAD21

Background: Name/Gene ID: RAD21

Synonyms: RAD21, CDLS4, HR21, KIAA0078, HHR21, NXP-1, NXP1, RAD21 (S. pombe) homolog, MCD1, Nuclear matrix protein 1, RAD21 homolog (S. pombe), HRAD21, SCC1, SCC1 homolog

Gene ID: 5885

NCBI Accession: NP_001020872

UniProt: O60216

Pathways: Positive Regulation of Endopeptidase Activity