PDYN antibody (AA 71-120)

Catalog No. ABIN6746613

The Rabbit Polyclonal anti-PDYN antibody has been validated for WB. It is suitable to detect PDYN in samples from Human, Rat and Mouse. There are 4+ publications available.

Quick Overview for PDYN antibody (AA 71-120) (ABIN6746613)

Target: PDYN (Prodynorphin (PDYN))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDYN antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PDYN Antibody

Binding Specificity: AA 71-120

Specificity: Human PDYN / Preprodynorphin

Predicted Reactivity: Percent identity by BLAST analysis: Mouse (100%) Rat (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa71-120 of mouse Pdyn (NP_061351). Percent identity by BLAST analysis: Mouse (100%), Rat, Hamster (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-PDYN antibody (ABIN6746613)

Morin, Awrey, Edwards, Arrowsmith: "Elongation factor TFIIS contains three structural domains: solution structure of domain II." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 20, pp. 10604-8, (1996) (PubMed).

Chen, England, Kane: "Characterization of a HeLa cDNA clone encoding the human SII protein, an elongation factor for RNA polymerase II." in: Gene, Vol. 116, Issue 2, pp. 253-8, (1992) (PubMed).

Agarwal, Baek, Jeon, Miyamoto, Ueno, Yoon: "Stimulation of transcript elongation requires both the zinc finger and RNA polymerase II binding domains of human TFIIS." in: Biochemistry, Vol. 30, Issue 31, pp. 7842-51, (1991) (PubMed).

Yoo, Yoon, Baek, Jeon, Miyamoto, Ueno, Agarwal: "Cloning, expression and characterization of the human transcription elongation factor, TFIIS." in: Nucleic acids research, Vol. 19, Issue 5, pp. 1073-9, (1991) (PubMed).

Target Details for PDYN

Target: PDYN (Prodynorphin (PDYN))

Alternative Name: PDYN / ProDynorphin

Background: Name/Gene ID: PDYN
Family: Endogenous opioids

Synonyms: PDYN, ADCA, Beta-neoendorphin-dynorphin, Leumorphin, Preprodynorphin, Spinocerebellar ataxia 23, PENKB, Prodynorphin, Rimorphin, SCA23, Leu-enkephalin, Preproenkephalin B, Proenkephalin-B

Gene ID: 5173

NCBI Accession: NP_061351

UniProt: P01213