ELMO1 antibody (AA 372-421)

Catalog No. ABIN6746630

The Rabbit Polyclonal anti-ELMO1 antibody has been validated for WB. It is suitable to detect ELMO1 in samples from Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Dog, Guinea Pig, Horse, Rabbit, Bat, Chicken, Hamster, Monkey, Pig and Xenopus laevis. There are 3+ publications available.

Quick Overview for ELMO1 antibody (AA 372-421) (ABIN6746630)

Target: ELMO1 (Engulfment and Cell Motility 1 (ELMO1))

Reactivity: Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Dog, Guinea Pig, Horse, Rabbit, Bat, Chicken, Hamster, Monkey, Pig, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ELMO1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ELMO1 Antibody

Binding Specificity: AA 372-421

Specificity: Human ELMO1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Horse, Pig, Chicken, Xenopus, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa372-421 of human ELMO1 (Q92556, NP_055615). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus, Salmon, Stickleback, Zebrafish (100%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-ELMO1 antibody (ABIN6746630)

Moro, Sirrenberg, Schneider, Neupert, Brunner: "The TIM17.23 preprotein translocase of mitochondria: composition and function in protein transport into the matrix." in: The EMBO journal, Vol. 18, Issue 13, pp. 3667-75, (1999) (PubMed).

Rassow, Dekker, van Wilpe, Meijer, Soll: "The preprotein translocase of the mitochondrial inner membrane: function and evolution." in: Journal of molecular biology, Vol. 286, Issue 1, pp. 105-20, (1999) (PubMed).

Ryan, Leung, Jensen: "Characterization of the mitochondrial inner membrane translocase complex: the Tim23p hydrophobic domain interacts with Tim17p but not with other Tim23p molecules." in: Molecular and cellular biology, Vol. 18, Issue 1, pp. 178-87, (1998) (PubMed).

Target Details for ELMO1

Target: ELMO1 (Engulfment and Cell Motility 1 (ELMO1))

Alternative Name: ELMO1 / ELMO 1

Background: Name/Gene ID: ELMO1

Synonyms: ELMO1, Ced-12 homolog 1, ELMO-1, Engulfment and cell motility 1, KIAA0281, Protein ced-12 homolog, CED-12, CED12

Gene ID: 9844

NCBI Accession: NP_055615

UniProt: Q92556