Fascin 2 antibody (AA 235-284)

Catalog No. ABIN6746674

This Rabbit Polyclonal antibody specifically detects Fascin 2 in WB. It exhibits reactivity toward Human, Rat, Guinea Pig, Rabbit and Zebrafish (Danio rerio) and has been mentioned in 4+ publications.

Quick Overview for Fascin 2 antibody (AA 235-284) (ABIN6746674)

Target: Fascin 2 (FSCN2)

Reactivity: Human, Rat, Guinea Pig, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Fascin 2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Fascin 2 Antibody

Binding Specificity: AA 235-284

Specificity: Human FSCN2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Rat, Guinea pig (100%) Mouse, Dog, Bovine, Horse (92%) Rabbit, Zebrafish (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa235-284 of human FSCN2 (O14926, NP_001070650). Percent identity by BLAST analysis: Human, Gorilla, Galago, Marmoset, Rat, Guinea pig (100%), Monkey, Mouse, Panda, Dog, Bovine, Horse (92%), Rabbit, Zebrafish (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 0.25 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-Fascin 2 antibody (ABIN6746674)

Saitoh, Pizzi, Wang: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, (2002) (PubMed).

Henglein, Chenivesse, Wang, Eick, Bréchot: "Structure and cell cycle-regulated transcription of the human cyclin A gene." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 12, pp. 5490-4, (1994) (PubMed).

Pines, Hunter: "The differential localization of human cyclins A and B is due to a cytoplasmic retention signal in cyclin B." in: The EMBO journal, Vol. 13, Issue 16, pp. 3772-81, (1994) (PubMed).

Pines: "Cyclins and cyclin-dependent kinases: take your partners." in: Trends in biochemical sciences, Vol. 18, Issue 6, pp. 195-7, (1993) (PubMed).

Target Details for Fascin 2

Target: Fascin 2 (FSCN2)

Alternative Name: FSCN2 / Retinal Fascin

Background: Name/Gene ID: FSCN2

Synonyms: FSCN2, Fascin-2, Retinal fascin, RP30, RFSN

Gene ID: 25794

NCBI Accession: NP_001070650

UniProt: O14926