MCEE antibody (AA 105-154)

Catalog No. ABIN6746806

The Rabbit Polyclonal anti-MCEE antibody is suitable to detect MCEE in samples from Human, Rat, Dog, Horse, Pig, Rabbit, Zebrafish (Danio rerio) and Monkey. It has been validated for WB.

Quick Overview for MCEE antibody (AA 105-154) (ABIN6746806)

Target: MCEE (Methylmalonyl CoA Epimerase (MCEE))

Reactivity: Human, Rat, Dog, Horse, Pig, Rabbit, Zebrafish (Danio rerio), Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCEE antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MCEE Antibody

Binding Specificity: AA 105-154

Specificity: Human MCEE

Predicted Reactivity: Percent identity by BLAST analysis: Human, Rat, Dog, Rabbit, Pig, Zebrafish (100%) Mouse, Bovine, Xenopus (92%) Chicken (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa105-154 of human MCEE (Q96PE7, NP_115990). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Rat, Elephant, Dog, Rabbit, Horse, Pig, Zebrafish (100%), Galago, Mouse, Bovine, Platypus, Xenopus, Frog, Stickleback, Pike (92%), Turkey, Zebra finch, Chicken (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for MCEE

Target: MCEE (Methylmalonyl CoA Epimerase (MCEE))

Alternative Name: GLOD2 / MCEE

Background: Name/Gene ID: MCEE

Synonyms: MCEE, DL-methylmalonyl-CoA racemase, GLOD2, Methylmalonyl CoA epimerase, Glyoxalase domain containing 2

Gene ID: 84693

NCBI Accession: NP_115990

UniProt: Q96PE7

Pathways: Monocarboxylic Acid Catabolic Process