HSDL1 antibody (C-Term)

Catalog No. ABIN6746873

The Rabbit Polyclonal anti-HSDL1 antibody has been validated for WB. It is suitable to detect HSDL1 in samples from Human, Rat, Guinea Pig, Monkey and Bat. There are 3+ publications available.

Quick Overview for HSDL1 antibody (C-Term) (ABIN6746873)

Target: HSDL1 (Hydroxysteroid Dehydrogenase Like 1 (HSDL1))

Reactivity: Human, Rat, Guinea Pig, Monkey, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSDL1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HSDL1 Antibody

Binding Specificity: C-Term

Specificity: Human HSDL1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Rat, Guinea pig (100%) Mouse, Dog, Bovine, Horse (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human HSDL1 (Q3SXM5, NP_113651). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Rat, Bat, Guinea pig (100%), Mouse, Elephant, Panda, Dog, Bovine, Horse, Pig (92%), Galago (91%), Rabbit (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-HSDL1 antibody (ABIN6746873)

Shain, Landowski, Dalton et al.: "Adhesion-mediated intracellular redistribution of c-Fas-associated death domain-like IL-1-converting enzyme-like inhibitory protein-long confers resistance to CD95-induced apoptosis in hematopoietic ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 5, pp. 2544-53, (2002) (PubMed).

Brown, Holden, Rahn, Perkins: "Immunohistochemical staining for DNA topoisomerase IIa in Hodgkin's disease." in: American journal of clinical pathology, Vol. 109, Issue 1, pp. 39-44, (1998) (PubMed).

Herzog, Holmes, Tuschong, Ganapathi, Zwelling: "Absence of topoisomerase IIbeta in an amsacrine-resistant human leukemia cell line with mutant topoisomerase IIalpha." in: Cancer research, Vol. 58, Issue 23, pp. 5298-300, (1998) (PubMed).

Target Details for HSDL1

Target: HSDL1 (Hydroxysteroid Dehydrogenase Like 1 (HSDL1))

Alternative Name: HSDL1

Background: Name/Gene ID: HSDL1

Synonyms: HSDL1, SDR12C3

Gene ID: 83693

NCBI Accession: NP_113651

UniProt: Q3SXM5