CD13 antibody (AA 142-191)

Catalog No. ABIN6746934

Product Details anti-CD13 Antibody

Target: CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))

Binding Specificity: AA 142-191

Reactivity: Human, Horse, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD13 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human ANPEP / CD13

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Horse (85%) Guinea pig (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa142-191 of human ANPEP (P15144, NP_001141). Percent identity by BLAST analysis: Human (100%), Gorilla, Gibbon, Monkey (92%), Galago, Elephant, Horse (85%), Opossum, Guinea pig (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-CD13 antibody (ABIN6746934)

Ohashi, Burkart, Flohé, Kolb: "Cutting edge: heat shock protein 60 is a putative endogenous ligand of the toll-like receptor-4 complex." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 2, pp. 558-61, (2000) (PubMed).

Xanthoudakis, Roy, Rasper, Hennessey, Aubin, Cassady, Tawa, Ruel, Rosen, Nicholson: "Hsp60 accelerates the maturation of pro-caspase-3 by upstream activator proteases during apoptosis." in: The EMBO journal, Vol. 18, Issue 8, pp. 2049-56, (1999) (PubMed).

Venner, Singh, Gupta: "Nucleotide sequences and novel structural features of human and Chinese hamster hsp60 (chaperonin) gene families." in: DNA and cell biology, Vol. 9, Issue 8, pp. 545-52, (1991) (PubMed).

Target Details for CD13

Target: CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))

Alternative Name: ANPEP / CD13 (ANPEP Products)

Background: Name/Gene ID: ANPEP
Subfamily: Metallopeptidase M1
Family: Exopeptidase

Synonyms: ANPEP, Alanyl aminopeptidase, Aminopeptidase M, APN, Aminopeptidase N, AP-N, AP-M, CD13, CD13 antigen, HAPN, gp150, p150, PEPN, Microsomal aminopeptidase, LAP1

Gene ID: 290

NCBI Accession: NP_001141

UniProt: P15144

Pathways: Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones