TTF1 antibody (AA 412-461)

Catalog No. ABIN6746964

This Rabbit Polyclonal antibody specifically detects TTF1 in WB. It exhibits reactivity toward Human and has been mentioned in 2+ publications.

Quick Overview for TTF1 antibody (AA 412-461) (ABIN6746964)

Target: TTF1 (Transcription Termination Factor, RNA Polymerase I (TTF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TTF1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TTF1 Antibody

Binding Specificity: AA 412-461

Specificity: Human TTF1

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa412-461 of human TTF1 (Q15361, NP_031370). Percent identity by BLAST analysis: Human, Gorilla (100%), Gibbon (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-TTF1 antibody (ABIN6746964)

Nagamine, Kudoh, Kawasaki, Minoshima, Asakawa, Ito, Shimizu: "Genomic organization and complete nucleotide sequence of the TMEM1 gene on human chromosome 21q22.3." in: Biochemical and biophysical research communications, Vol. 235, Issue 1, pp. 185-90, (1997) (PubMed).

Scott, Chen, Rossier, Lalioti, Antonarakis: "Isolation of a human gene (HES1) with homology to an Escherichia coli and a zebrafish protein that maps to chromosome 21q22.3." in: Human genetics, Vol. 99, Issue 5, pp. 616-23, (1997) (PubMed).

Target Details for TTF1

Target: TTF1 (Transcription Termination Factor, RNA Polymerase I (TTF1))

Alternative Name: TTF1 / Txn Termination Factor

Background: Name/Gene ID: TTF1

Synonyms: TTF1, TTF-I, TTF-1

Gene ID: 7270

NCBI Accession: NP_031370

UniProt: Q15361

Pathways: Thyroid Hormone Synthesis, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour