SIRT4 antibody (AA 216-265)

Catalog No. ABIN6746987

The Rabbit Polyclonal anti-SIRT4 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect SIRT4 in samples from Human, Dog, Pig and Monkey.

Quick Overview for SIRT4 antibody (AA 216-265) (ABIN6746987)

Target: SIRT4 (Sirtuin 4 (SIRT4))

Reactivity: Human, Dog, Pig, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIRT4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SIRT4 Antibody

Binding Specificity: AA 216-265

Brand: IHC-plus™

Specificity: Human SIRT4

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Mouse, Rat, Sheep, Bovine (92%) Pig (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa216-265 of human SIRT4 (Q9Y6E7, NP_036372). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Galago, Marmoset (100%), Mouse, Rat, Sheep, Elephant, Bovine, Rabbit (92%), Pig (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for SIRT4

Target: SIRT4 (Sirtuin 4 (SIRT4))

Alternative Name: SIRT4 / Sirtuin 4

Background: Name/Gene ID: SIRT4
Family: ADP-Ribosyltransferase

Synonyms: SIRT4, Sir2-like 4, SIR2-like protein 4, Sirtuin 4, Sirtuin type 4, SIR2L4

Gene ID: 23409

NCBI Accession: NP_036372

UniProt: Q9Y6E7

Pathways: Negative Regulation of Hormone Secretion