GJB1 antibody (C-Term)

Catalog No. ABIN6747019

This Rabbit Polyclonal antibody specifically detects GJB1 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Rat, Mouse, Dog, Cow, Horse, Rabbit, Monkey, Pig, Bat and Hamster.

Quick Overview for GJB1 antibody (C-Term) (ABIN6747019)

Target: GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))

Reactivity: Human, Rat, Mouse, Dog, Cow, Horse, Rabbit, Monkey, Pig, Bat, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GJB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GJB1 Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Human GJB1 / Connexin 32

Predicted Reactivity: Percent identity by BLAST analysis: Mouse, Dog (100%) Guinea pig (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human GJB1 (P08034, NP_000157). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig (100%), Guinea pig (92%), Platypus (86%), Opossum (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for GJB1

Target: GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))

Alternative Name: GJB1 / CX32 / Connexin 32

Background: Name/Gene ID: GJB1
Subfamily: Connexin
Family: Ion Channel

Synonyms: GJB1, Connexin 32, CMTX1, Connexin-32, CX32, Gap junction beta-1 protein, Gap junction protein beta-1, CMTX

Gene ID: 2705

NCBI Accession: NP_000157

UniProt: P08034

Pathways: Cell-Cell Junction Organization