KLF4 antibody (AA 401-450)

Catalog No. ABIN768780

This anti-KLF4 antibody is a Rabbit Polyclonal antibody detecting KLF4 in WB, IHC and IHC (p). Suitable for Human, Mouse, Rat, Cow, Horse, Dog, Rabbit, Sheep, Pig, Monkey, Guinea Pig, Bat, Zebrafish (Danio rerio), Hamster, Chicken, Drosophila melanogaster and Xenopus laevis.

Quick Overview for KLF4 antibody (AA 401-450) (ABIN768780)

Target: KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))

Reactivity: Human, Mouse, Rat, Cow, Horse, Dog, Rabbit, Sheep, Pig, Monkey, Guinea Pig, Bat, Zebrafish (Danio rerio), Hamster, Chicken, Drosophila melanogaster, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KLF4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-KLF4 Antibody

Binding Specificity: AA 401-450

Specificity: Human KLF4

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Sheep, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Xenopus, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa401-450 of human KLF4 (O43474, NP_004226). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Hamster, Elephant, Panda, Dog, Cat, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus, Salmon, Stickleback, Zebrafish, Drosophila (100%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for KLF4

Target: KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))

Alternative Name: KLF4

Background: Name/Gene ID: KLF4

Synonyms: KLF4, EZF, GKLF, Kruppel-like factor 4 (gut), Hezf, Krueppel-like factor 4

Gene ID: 9314

NCBI Accession: NP_004226

UniProt: O43474

Pathways: Peptide Hormone Metabolism, Stem Cell Maintenance