FZD5 antibody (AA 467-516)

Catalog No. ABIN6747065

The Rabbit Polyclonal anti-FZD5 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect FZD5 in samples from Human, Mouse, Rat and Cow.

Quick Overview for FZD5 antibody (AA 467-516) (ABIN6747065)

Target: FZD5 (Frizzled Family Receptor 5 (FZD5))

Reactivity: Human, Mouse, Rat, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FZD5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-FZD5 Antibody

Binding Specificity: AA 467-516

Specificity: Human FZD5 / Frizzled 5

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Mouse, Rat, Bovine (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa467-516 of human FZD5 (Q13467, NP_003459). Percent identity by BLAST analysis: Human (100%), Gorilla (92%), Gibbon, Monkey, Galago, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Pig, Guinea pig (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for FZD5

Target: FZD5 (Frizzled Family Receptor 5 (FZD5))

Alternative Name: FZD5 / Frizzled 5

Background: Name/Gene ID: FZD5
Subfamily: Frizzled
Family: GPCR

Synonyms: FZD5, C2orf31, DKFZP434E2135, Frizzled family receptor 5, Fz5, Fzd-5, FzE5, Frizzled 5, Frizzled-5, Wnt receptor, Fz-5, HFZ5

Gene ID: 7855

NCBI Accession: NP_003459

UniProt: Q13467

Pathways: WNT Signaling