ROBO2 antibody (AA 252-301)

Catalog No. ABIN6747098

The Rabbit Polyclonal anti-ROBO2 antibody (ABIN6747098) specifically detects ROBO2 in WB, IHC and IHC (p). The antibody is reactive with Human, Mouse, Rat, Cow, Chicken, Dog, Horse, Rabbit, Zebrafish (Danio rerio), Monkey and Pig samples.

Quick Overview for ROBO2 antibody (AA 252-301) (ABIN6747098)

Target: ROBO2 (Roundabout, Axon Guidance Receptor, Homolog 2 (ROBO2))

Reactivity: Human, Mouse, Rat, Cow, Chicken, Dog, Horse, Rabbit, Zebrafish (Danio rerio), Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ROBO2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ROBO2 Antibody

Binding Specificity: AA 252-301

Specificity: Human ROBO2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Chicken (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa252-301 of human ROBO2 (Q9HCK4, NP_002933). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Zebra finch, Chicken, Platypus (100%), Bat (92%), Opossum (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ROBO2

Target: ROBO2 (Roundabout, Axon Guidance Receptor, Homolog 2 (ROBO2))

Alternative Name: ROBO2

Background: Name/Gene ID: ROBO2

Synonyms: ROBO2, KIAA1568, Roundabout homolog 2, SAX3

Gene ID: 6092

NCBI Accession: NP_002933

UniProt: Q9HCK4

Pathways: Regulation of Cell Size