NudE Neurodevelopment Protein 1 (NDE1) (AA 218-267) antibody

Catalog No. ABIN6747113

This anti- antibody is a Rabbit Polyclonal antibody detecting in WB, IHC and IHC (p). Suitable for Human, Mouse, Rat, Chicken, Cow, Dog, Guinea Pig, Hamster, Horse, Monkey and Rabbit.

Quick Overview for NudE Neurodevelopment Protein 1 (NDE1) (AA 218-267) antibody (ABIN6747113)

Target: NudE Neurodevelopment Protein 1 (NDE1)

Reactivity: Human, Mouse, Rat, Chicken, Cow, Dog, Guinea Pig, Hamster, Horse, Monkey, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details

Binding Specificity: AA 218-267

Brand: IHC-plus™

Specificity: Human NDE1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Horse, Guinea pig, Chicken (100%) Xenopus (92%) Zebrafish (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa218-267 of human NDE1 (Q9NXR1-2, NP_060138). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Rabbit, Horse, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus (100%), Orangutan, Galago, Bat, Pig, Xenopus, Zebrafish (92%), Lizard (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details

Target: NudE Neurodevelopment Protein 1 (NDE1)

Alternative Name: NDE1 / NUDE

Background: Name/Gene ID: NDE1

Synonyms: NDE1, HOM-TES-87, LIS4, NUDE, NUDE1

Gene ID: 54820

NCBI Accession: NP_060138

UniProt: Q9NXR1

Pathways: M Phase