NudE Neurodevelopment Protein 1 (NDE1) (AA 218-267) antibody

Catalog No. ABIN6747113

The Rabbit Polyclonal anti-NudE Neurodevelopment Protein 1 antibody (ABIN6747113) specifically detects NudE Neurodevelopment Protein 1 in WB, IHC and IHC (p). The antibody is reactive with Human, Mouse, Rat, Chicken, Cow, Dog, Guinea Pig, Hamster, Horse, Monkey and Rabbit samples.

Quick Overview for NudE Neurodevelopment Protein 1 (NDE1) (AA 218-267) antibody (ABIN6747113)

Target: NudE Neurodevelopment Protein 1 (NDE1)

Reactivity: Human, Mouse, Rat, Chicken, Cow, Dog, Guinea Pig, Hamster, Horse, Monkey, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details

Binding Specificity: AA 218-267

Specificity: Human NDE1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Horse, Guinea pig, Chicken (100%) Xenopus (92%) Zebrafish (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa218-267 of human NDE1 (Q9NXR1-2, NP_060138). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Rabbit, Horse, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus (100%), Orangutan, Galago, Bat, Pig, Xenopus, Zebrafish (92%), Lizard (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details

Target: NudE Neurodevelopment Protein 1 (NDE1)

Alternative Name: NDE1 / NUDE

Background: Name/Gene ID: NDE1

Synonyms: NDE1, HOM-TES-87, LIS4, NUDE, NUDE1

Gene ID: 54820

NCBI Accession: NP_060138

UniProt: Q9NXR1

Pathways: M Phase