PGK1 antibody (AA 102-151)

Catalog No. ABIN6747118

The Rabbit Polyclonal anti-PGK1 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect PGK1 in samples from Human, Mouse, Rat, Cow, Sheep, Dog, Guinea Pig, Horse, Rabbit, Monkey and Bat.

Quick Overview for PGK1 antibody (AA 102-151) (ABIN6747118)

Target: PGK1 (Phosphoglycerate Kinase 1 (PGK1))

Reactivity: Human, Mouse, Rat, Cow, Sheep, Dog, Guinea Pig, Horse, Rabbit, Monkey, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PGK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PGK1 Antibody

Binding Specificity: AA 102-151

Brand: IHC-plus™

Specificity: Human PGK1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Sheep, Dog, Bovine, Horse, Guinea pig (100%) Pig, Chicken, Xenopus, Zebrafish (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa102-151 of human PGK1 (P00558, NP_000282). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Panda, Dog, Bovine, Bat, Rabbit, Horse, Guinea pig (100%), Hamster, Elephant, Pig, Opossum, Zebra finch, Chicken, Platypus, Xenopus, Salmon, Stickleback, Zebrafish, Mosquito (92%), Medaka, Drosophila (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 0.5 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PGK1

Target: PGK1 (Phosphoglycerate Kinase 1 (PGK1))

Alternative Name: PGK1/Phosphoglycerate Kinase 1

Background: Name/Gene ID: PGK1
Subfamily: PGK
Family: Non-protein Kinase

Synonyms: PGK1, MIG10, Primer recognition protein 2, PRP 2, PGKA, Phosphoglycerate kinase 1

Gene ID: 5230

NCBI Accession: NP_000282

UniProt: P00558

Pathways: Cellular Glucan Metabolic Process