GCLC antibody (AA 74-123)

Catalog No. ABIN6747154

This Rabbit Polyclonal antibody specifically detects GCLC in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Rabbit, Monkey, Dog, Guinea Pig, Pig, Bat and Hamster.

Quick Overview for GCLC antibody (AA 74-123) (ABIN6747154)

Target: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Reactivity: Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Rabbit, Monkey, Dog, Guinea Pig, Pig, Bat, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GCLC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GCLC Antibody

Binding Specificity: AA 74-123

Brand: IHC-plus™

Specificity: Human GCLC

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Dog, Bovine, Rabbit, Pig, Guinea pig (100%) Horse (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa74-123 of human GCLC (Q14TF0, NP_001489). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Pig, Guinea pig (100%), Gibbon, Horse, Lizard (92%), Opossum, Platypus, Salmon (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for GCLC

Target: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Alternative Name: GCLC

Background: Name/Gene ID: GCLC

Synonyms: GCLC, GCL, GCS heavy chain, Gamma-ECS, GCS, GLCL, GLCLC

Gene ID: 2729

NCBI Accession: NP_001489

UniProt: P48506

Pathways: Cell RedoxHomeostasis