HINT1 antibody (N-Term)

Catalog No. ABIN6747162

This anti-HINT1 antibody is a Rabbit Polyclonal antibody detecting HINT1 in WB, IHC and IHC (p). Suitable for Human, Mouse, Rat, Cow, Dog, Rabbit, Pig, Zebrafish (Danio rerio), Chicken, Monkey and Xenopus laevis.

Quick Overview for HINT1 antibody (N-Term) (ABIN6747162)

Target: HINT1 (Histidine Triad Nucleotide Binding Protein 1 (HINT1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Pig, Zebrafish (Danio rerio), Chicken, Monkey, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HINT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HINT1 Antibody

Binding Specificity: N-Term

Brand: IHC-plus™

Specificity: Human HINT1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Chicken, Xenopus, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human HINT1 (P49773, NP_005331). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Rabbit, Pig, Zebra finch, Chicken, Lizard, Xenopus, Catfish, Salmon, Sablefish, Stickleback, Zebrafish (100%), Gibbon, Opossum (92%), Drosophila, Nematode (90%), Beetle (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for HINT1

Target: HINT1 (Histidine Triad Nucleotide Binding Protein 1 (HINT1))

Alternative Name: HINT / HINT1

Background: Name/Gene ID: HINT1

Synonyms: HINT1, HINT, NMAN, Protein kinase C inhibitor 1, PKCI-1, PKCI1, PRKCNH1

Gene ID: 3094

NCBI Accession: NP_005331

UniProt: P49773