HINT1 antibody (AA 38-87)

Catalog No. ABIN6747163

This Rabbit Polyclonal antibody specifically detects HINT1 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Rabbit, Horse, Pig, Guinea Pig, Chicken and Monkey.

Quick Overview for HINT1 antibody (AA 38-87) (ABIN6747163)

Target: HINT1 (Histidine Triad Nucleotide Binding Protein 1 (HINT1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Horse, Pig, Guinea Pig, Chicken, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HINT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HINT1 Antibody

Binding Specificity: AA 38-87

Brand: IHC-plus™

Specificity: Human HINT1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Guinea pig, Chicken (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa38-87 of human HINT1 (P49773, NP_005331). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Zebra finch, Chicken (100%), Lizard (92%), Drosophila (91%), Opossum, Rice, Nematode, Grape (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for HINT1

Target: HINT1 (Histidine Triad Nucleotide Binding Protein 1 (HINT1))

Alternative Name: HINT / HINT1

Background: Name/Gene ID: HINT1

Synonyms: HINT1, HINT, NMAN, Protein kinase C inhibitor 1, PKCI-1, PKCI1, PRKCNH1

Gene ID: 3094

NCBI Accession: NP_005331

UniProt: P49773